Basic Information

Reference Number

0000254492

Issuing Organization

University of Ottawa

Owner Organization

University of Ottawa

Solicitation Type

RFP - Request for Proposal (Formal)

Solicitation Number

36490-39A-RFP

Title

Pre-clinical optical in-vivo imaging system (IVIS)

Source ID

PU.AC.ON.11752.C84282

Agreement Type

Broader Public Sector (BPS)

Details

Location

Canada, Ontario, Ottawa

Delivery Point

Ottawa

Purchase Type

One Time Only- Delivery Date:TBD

Description

PART 1: INTRODUCTION

1. - Background Information

Since 1848 the University of Ottawa has been Canada’s university and the largest bilingual university in Canada. At the University of Ottawa, we understand our past and our desired future. The University of Ottawa is a major player in the cultural and economic development of the National Capital Region.

The University of Ottawa is one of Canada's leading research-intensive University. The University of Ottawa fosters excellence and success in research, whether the research is basic or applied, of intrinsic value or of commercial importance. The University of Ottawa considers itself to be Canada's university of the 21st Century by launching new initiatives in emerging areas, promoting collaboration within and across faculties, and providing the milieu in which creativity can flourish.

Further information regarding the University of Ottawa can be found on the Web site http://www.uottawa.ca/

We hereby invite your company (proponent) to partner with the University of Ottawa in this great project funded by the Canada Foundation for Innovation and the Ontario Research Fund by submitting an offer to this Request for Proposals and by including an in-kind contribution.

Please find attached the Request for Proposals documentation with specifications and the CFI guidelines pricing format to be completed and returned with your proposal.

1. - Statement of Requirements

1.2.1.       The Unversity of Ottawa is pleased to invite qualified proponents to submit a proposal for pre-clinical optical in-vivo imaging system (IVIS) to allow the evaluation of cell- and gene-based diseases & therapies using fluorescent, or luminescent tracing methods.

1.2.2        Scope of Project:

The requested optical in-vivo imaging system (IVIS) is a critical piece of infrastructure required to allow the evaluation of cell- and gene-based diseases & therapies using fluorescent, or luminescent tracing methods in small animals (e.g., mice, rats, guinea pigs, small rabbits, and zebra fishes) in-vivo, ex-vivo specimens (e.g., organs and tissue sections) and in vitro applications (in microplates, petri-dishes, etc.). The IVIS is a necessary tool in oncology, virology, neuroscience, biodistribution studies, treatment studies, cell tracking, and ex-vivo imaging.

The equipment will enable state-of-the-art approaches e.g., to visualize and track tumours & infections, diseases, or inflammation development, or follow biodistribution and pharmacokinetics studies, or therapy progression within living animals in a non-invasive manner. In particular it will be used for the development of human rodent disease models, assess dysfunction in models of disease, and the evaluation of cell- and gene-based therapies using fluorescent, or luminescent labelling/tracing methods.

1.3           General Description of Instrument/Product, System or Service:

The in-vivo imaging system is a non-invasive visualization tool to study living organisms or samples for research or diagnostic purposes. The two main principles used are bioluminescence and fluorescence imaging.

Bioluminescence imaging is based on the reaction between a reporter gene (luciferase) and its substrate (D-luciferin). The injected substrate reacts with the reporter gene and light is emitted. Fluorescence imaging, on the contrary, is based on excitation of a fluorophore which subsequently returns to its original energetic state producing photons with higher wavelengths.

Various bioluminescent reporters like a firefly luciferase and many fluorescent molecular reagents can be used to visualize and track tumours, disease or inflammation development, target molecules to nanoparticles or follow biodistribution and pharmacokinetics studies within living animals in a noninvasive manner.

1.4          Specifications and Standards:

The proposed system must be capable of performing in-vivo, ex-vivo and in-vitro imaging of small animals and detect fluorescent and bioluminescent sources.

Camera & Optics
The CCD camera operating temperature must be -90°C or lower.
To capture as much light as possible the lens aperture must allow f/stops being f/1.2 or lower (e.g., f/0.90) .
The peak quantum efficiency (QE) must be at least 80% within the range 500 – 700nm.
The native camera resolution must be at least 2000x2000 or higher with a pixel size of 15mm or lower (e.g., 8.0mm).
The camera must be a 16-bit CCD Camera with high optical density.
The proposed system must be calibrated to a NIST standard.
Field of view (FOV): the proposed system must be capable of imaging up to min 5 mice or 2 rats at a time as a standard across all modalities and allow macro imaging.

Illumination
The proposed system must include min 8 excitation channels from ~400 to ~800nm
The proposed system must include min 8 narrow bandpass emission filters from ~500 to ~900nm
The proposed system must allow visualizing several reporters simultaneously i.e., multi-spectral capabilities and spectral unmixing features for both bioluminescence and fluorescence imaging.

Hardware
The proposed system must have a fully software-controlled motorized system for lens, camera, and sample stage.

Animal Management
The proposed system must have a heated platform.
The proposed system must have an integrated gas anesthesia platform with an animal breather up to min 5 mice.
The proposed system must have a gas scavenging platform.

3D Imaging
The proposed system must provide 3D mapping of the target object for 3D source signal volume reconstruction allowing labelling and multimodal fusion.

Specifications listed in Appendix A – Technical Specifications Compliance Form of this RFP are the mandatory minimum requirements for the pre-clinical optical in-vivo imaging system (IVIS).

1.5           Should any equipment included in a proposal be superseded by a newer model during the period between the award of contract and the delivery date, the successful Proponent will be required to inform the University of Ottawa of the new specifications, the newer model pricing will remain the same as what was offered in the RFP. At its sole discretion, the University of Ottawa may opt to make changes from the list of equipment by altering, adding, or deducting some equipment.

Dates

Publication

2023/08/18 03:04:05 PM EDT

Bid Intent

Not Available

Question Acceptance DeadlineA - Previous Amendment

2023/08/29 03:00:00 PM EDT

Questions are submitted online

Yes

Closing DateA - Latest Amendment

2023/09/06 03:00:00 PM EDT

Contact Information

Roger Wills

16135625800 ext. 7113

rwills@uottawa.ca

Bid Submission Process

Bid Submission Type

Electronic Bid Submission

Pricing

In attached document

Bid Documents List

Item Name	Description	Mandatory	Limited to 1 file
Attachment # 1	Technical/Qualitative Proposal: 36490-39a-RFP Pre-clinical optical in-vivo imaging system (IVIS)	Yes	No
Attachment # 2	Financial Proposal: 36490-39a-RFP Pre-clinical optical in-vivo imaging system (IVIS)	No	No
No entries

Additional Bidding Instructions

1. - Proposals Submitted Only In Prescribed Manner

1. 1. The Proponent’s proposal must be submitted in the following manner:

1. 1. The Proponents must submit their proposal using Merx EBS no later than the time and date stated in section 2.2 – Timetable. No other forms of delivery will be accepted by the University.

The proposals must be complete and submitted in the prescribed format with the following information:

1. 1. The Proponent must provide two (2) separate attachments (files) containing the Proponent’s proposal labelled in the following manner:

Attachment #1 Technical/Qualitative Proposal: 36490-39a-RFP Pre-clinical optical in-vivo imaging system (IVIS)

Attachment #2 Financial Proposal: 36490-39a-RFP Pre-clinical optical in-vivo imaging system (IVIS)

Attachment #1 – Technical/Qualitative Proposal must include the following mandatory documents:

Appendix A (Technical Specifications Compliance)
Appendix B (Rated Criteria)
Appendix C (Equipment, Utility, and Environmental Requirements) and all the referenced associated documents
Appendix D (Supplemental Information)
Appendix E (Reference Consent Form)
Appendix F (Submission Declaration Form)
Appendix I (Proponent’s Checklist Form)
All supporting technical documentation for the proposal
Detailed quotation without pricing
Bottom of every page of RFP initialled

1. 1. Attachment #2 – Financial Proposal must include the following mandatory documents:

Appendix G (Pricing Structure Form)

Appendix H (Price Schedule Form)
Detailed quotation with pricing

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